Terminal galactosylation of glycoconjugates in Plasmodium falciparum asexual blood stages and Trypanosoma brucei bloodstream trypomastigotes

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• 作者：Ranjan Ramasamy^a ; ^b ; ^{ranjan.ramasamy@ubd.edu.bn} ; Mark C. Field^a
• 关键词：Bandeirea (Griffonia) simplicifolia B4 lectin 1 ; Galactosylation ; Glycoconjugate ; Plasmodium falciparum ; Trypanosoma brucei
• 刊名：Experimental Parasitology
• 出版年：2012
• 期刊代码：99_00144894
• 类别：imm
• 出版时间：April, 2012
• 卷：130
• 期：4
• 页码：314-320
• 文件大小：370 K

摘要

There is definitive biochemical evidence for the presence of terminal 伪-galactosyl residues (伪-gal) in the N-linked oligosaccharides and glycophosphatidylinositol anchors (GPI anchors) of the variant surface glycoprotein of Trypanosoma brucei bloodstream trypomastigotes. Indirect evidence also exists for 伪-gal in Plasmodium falciparum asexual blood stage glycoproteins and glycolipids. The occurrence of 伪-gal in glycoproteins and glycolipids of T. brucei bloodstream trypomastigotes and P. falciparum late asexual blood stages was investigated by the binding of 伪-gal-specific Bandeirea simplicifolia B4 lectin 1 (BSB4), incorporation of [³H]galactose from UDP-[³H]galactose into glycoproteins and glycolipids in microsomes in vitro, and bioinformatic searches for galactosyl-transferase coding sequences. The findings confirm the presence of 伪-gal in a spectrum of T. brucei bloodstream trypomastigote glycoproteins and glycolipids and indicate its relative absence from P. falciparum asexual blood stage glycoconjugates.