Differential effects of the iodinated contrast agents Ioxaglate, Iohexol and Iodixanol on thrombus formation and fibrinolysis
- 作者：Jones ; Chris I. ; Goodall ; Alison H.
- 关键词：Contrast media ; Thrombi ; Fibrinolysis ; Platelets
- 刊名：Thrombosis Research
- 出版年：2003
- 期刊代码：247_00493848
- 类别：med
- 出版时间：2003
- 卷：112
- 期：1-2
- 页码：65-71
- 文件大小：577 K
摘要
Introduction: Contrast media (CM) possess both pro-thrombotic and anticoagulant properties. Here we investigate the effect of three classes of CM; Iohexol, Iodixanol and Ioxaglate, on thrombus formation and fibrinolysis in vitro and evaluated the contribution of platelets to this process. Materials and methods: Non-anticoagulated blood was mixed with CM or saline (50%or 20%(v/v)) for 1 min then citrated. Thrombi were produced in vitro under flow in a Chandler loop. Thrombus structure was visualized by immunohistochemistry using FITC-fibrin/ogen antibodies, and propidium iodide to identify nucleated blood cells. To measure fibrino/thrombolysis release of FITC-labeled fibrinogen, added prior to thrombus formation, was measured over 24 h. Platelet degranulation was analyzed by whole blood flow-cytometry. Results: No thrombi formed from blood incubated with Ioxaglate. Thrombi formed with Iohexol or Iodixanol weighed ≥10× more than saline controls (116±52 and 230±128 mg vs. 11±3 mg, respectively; p<0.0005), and were more resistant to thrombolysis as evidenced by the release of FITC over 24 h (19.1±8.9 and 31.9±17.2 U vs. 65.1±19.1 U, respectively; p<0.02). Thrombi formed with Iodixanol and Iohexol had larger, more diffuse platelet-rich head areas and tail regions composed of a more open fibrinogen/fibrin meshwork enclosing denser RBC rich areas compared to controls. Iodixanol and Ioxaglate did not increase platelet degranulation, but Iohexol caused a significant increase compared to the saline control (percentage of platelets expressing P-selectin 68.9±23.43%vs. 4.93±1.44%, respectively; p