Alimentary lipemia enhances the membrane expression of platelet P-selectin without affecting other markers of platelet activation
- 作者：Brö ; ijersé ; n ; Anders ; Karpe ; Fredrik ; Hamsten ; Anders ; Goodall ; Alison H. ; Hjemdahl ; Paul
- 关键词：Alimentary lipemia ; Platelets ; Filtragometry ; Flow cytometry ; P-selectin ; β ; -Thromboglobulin ; Platelet factor 4
- 刊名：Atherosclerosis
- 出版年：1998
- 期刊代码：28_00219150
- 类别：med
- 出版时间：March, 1998
- 卷：137
- 期：1
- 页码：107-113
- 文件大小：612 K
摘要
The present study was conducted to determine whether alimentary lipemia alters platelet activity in vivo. Normolipidemic volunteers were given a fatty meal and platelet function was assessed before, and 3 and 6 h after the meal. Platelet aggregability and secretion was determined using whole blood flow cytometry (expression of platelet P-selectin and fibrinogen binding), filtragometry ex vivo (reflecting platelet aggregability in vivo) and by measurements of platelet specific products in plasma (β-thromboglobulin and platelet factor 4). Plasma triglycerides increased from 0.8 (0.6:1.1; median, 25th and 75th percentiles) to 1.7 (1.0:2.3) mmol/l at 3 h and returned to baseline after 6 h (P < 0.001, one-way ANOVA). Apo B-100 and apo B-48 were both markedly increased 3 h postprandially in the Sf 60-400 fraction (large VLDLs, P < 0.001 for both), whereas the Sf 20-60 (small VLDLs) and Sf 12-20 fractions (IDL) did not change. The platelet function assessments revealed that the percentage of platelets expressing P-selectin increased by 40%(5%; 64%) after 3 h and by 51%(− 7%; 85%) 6 h postprandially in unstimulated samples (P < 0.05 for both). In samples stimulated by ADP in vitro P-selectin expression increased by 45%(6%; 58%) after 3 h and by 30%(12%; 58%) (P < 0.01 for both) after 6 h at 0.1 μM. Platelet P-selectin expression was less influenced at higher ADP concentrations. The plasma levels of β-thromboglobulin ( 20 ng/ml) and platelet factor 4 ( 0.3 ng/ml) were not affected by the fat load. Flow cytometric analyses of fibrinogen binding and filtragometry measurements also failed to reveal any postprandial alterations. The present finding of enhanced platelet P-selectin expression suggests that platelets are mildly sensitized postprandially. Whether this is of importance for thrombus formation and atherosclerosis needs to be studied further.