Determination of reduced glutathione using an amperometric carbon paste electrode chemically modified with TTF–TCNQ

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• 作者：Calvo-Marzal ; Percy ; Chumbimuni-Torres ; Karin Y. ; Hö ; ehr ; Nelci Fenalti ; de Oliveira Neto ; Graciliano ; et. al.
• 关键词：Reduced glutathione ; Modified electrode ; TTF&ndash ; TCNQ
• 刊名：Sensors and Actuators B ; Chemical
• 出版年：2004
• 期刊代码：161_09254005
• 类别：et
• 出版时间：May 15, 2004
• 卷：100
• 期：3
• 页码：337-344
• 文件大小：149 K

摘要

The development of an amperometric sensor for the determination of reduced glutathione (GSH) is described. The sensor is based on tetrathiafulvalene–tetracyanoquinodimethane (TTF–TCNQ) incorporated into the graphite powder/Nujol oil matrix. The electrooxidation of GSH was monitored amperometrically at 200mV versus SCE (saturated calomel electrode). The amperometric response of the sensor was linearly proportional to the GSH concentration between 20 and 300μmoll⁻¹, in 0.1moll⁻¹ phosphate buffer (pH 8.0), containing 0.1moll⁻¹ KCl and 0.5mmoll⁻¹ Na₂H₂EDTA, as supporting electrolyte.