Baculovirus cDNA libraries for expression cloning of genes encoding cell-surface antigens

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• 作者：Granziero ; Luisa ; Nelboeck ; Peter ; Bedoucha ; Marc ; Lanzavecchia ; Antonio ; Reid ; Hugh H.
• 关键词：Expression cloning ; Baculovirus ; Surface antigen
• 刊名：Journal of Immunological Methods
• 出版年：1997
• 期刊代码：57_00221759
• 类别：imm
• 出版时间：April 25, 1997
• 卷：203
• 期：2
• 页码：131-139
• 文件大小：641 K

摘要

We describe a method for the production of baculovirus-based cDNA libraries. By staining with monoclonal antibodies, single positive cells can be sorted and the virus encoding for the surface epitope can be isolated by limiting dilution. We have used this method to isolate cDNAs encoding several cell-surface antigens.