Oxidative stress and genotoxic responses to resin acids in Mediterranean mussels
- 作者：Gravato ; C. ; Oliveira ; M. ; Santos ; M.A.
- 关键词：Abietic acid ; Dehydroabietic acid ; Resin acids ; Enzymatic and nonenzymatic antioxidants ; Lipid peroxidation ; DNA strand breaks ; Mytilus galloprovincialis
- 刊名：Ecotoxicology and Environmental Safety
- 出版年：2005
- 期刊代码：77_01476513
- 类别：env
- 出版时间：June, 2005
- 卷：61
- 期：2
- 页码：221-229
- 文件大小：271 K
摘要
This study represents the first attempt to investigate the genotoxic effects and oxidative stress of resin acids in Mediterranean mussels (Mytilus galloprovincialis Lmk.). Mussels were exposed to 2.7 ;c;M abietic acid (AA) and dehydroabietic acid (DHAA) for 6, 12, 18, and 24 h. Gill and hepatopancreas conjugation activity, antioxidant defense system, lipid peroxidation (LPO), and DNA damage were determined as reduced glutathione (GSH), glutathione S-transferase (GST) activity, glutathione peroxidase (GPx) activity, catalase (CAT) activity, LPO, and DNA strand breaks. AA caused significant GST inhibition in mussel gills at 12, 18, and 24 h. Activity of the antioxidant enzymes, namely, GPx and CAT, was inhibited at 24 and 18 h, respectively, in mussel gills. A significant increase in gill LPO was observed at 24 h. The DNA integrity of mussel hepatopancreas significantly decreased after 12 and 24 h exposure to AA. A significant increase in LPO was observed after 6 h exposure to DHAA, in either mussel gills or hepatopancreas. DNA integrity was significantly decreased in mussel hepatopancreas after 12 and 24 h exposure to DHAA. AA induced oxidative damage and genotoxicity in mussels, because it promoted increases in LPO in gills and DNA strand breaks in hepatopancreas. DHAA promoted oxidative damage and genotoxicity in mussels, as significant increases were observed in LPO in gills and hepatopancreas and in DNA strand breaks in hepatopancreas.