Adsorption behaviour of hen egg-white lysozyme at the air/water interface
- 作者：V.S. Alahverdjieva ; D.O. Grigoriev ; J.K. Ferri ; V.B. Fainerman ; E.V. Aksenenko ; M.E. Leser ; M. Michel ; R. Miller
- 关键词：Adsorption of proteins ; Solution/air interface ; Thermodynamic model ; Lysozyme
- 刊名：Colloids and Surfaces A ; Physicochemical and Engineering Aspects
- 出版年：2008
- 期刊代码：22_09277757
- 类别：ch
- 出版时间：20 June 2008
- 卷：323
- 期：1-3
- 页码：167-174
- 文件大小：347 K
摘要
The adsorption dynamics and equilibrium properties of hen egg-white lysozyme at the air/water interface is studied using bubble and drop profile analysis technique, ellipsometry and infrared reflection absorption spectroscopy (IRRAS). The set of equilibrium data can be described by a recently developed thermodynamic model. Using the equilibrium adsorption parameters the adsorption dynamics can be well described by a diffusion controlled mechanism at the lower concentrations and a mixed model at higher lysozyme concentrations where conformational changes in the surface layer become time determining.