Ethanol suppresses phagosomal adhesion maturation, Rac activation, and subsequent actin polymerization during Fc纬R-mediated phagocytosis

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• 作者：John Karavitis^a ; ^b ; ^c ; Eva L. Murdoch^a ; ^b ; ^c ; Cory Deburghgraeve^a ; ^c ; ^d ; Luis Ramirez^b ; ^c ; ^d ; Elizabeth J. Kovacs^b ; ^c ; ^d ; ^{ekovacs@lumc.edu}
• 关键词：Alcohol ; Phagosome ; Small GTPase ; Rac ; Rho ; VavGEF ; Alveolar macrophage ; RAW264.7
• 刊名：Cellular Immunology
• 出版年：2012
• 期刊代码：279_00088749
• 类别：med
• 出版时间：2012
• 卷：274
• 期：1-2
• 页码：61-71
• 文件大小：844 K

摘要

Clinical and laboratory investigations have provided evidence that ethanol suppresses normal lung immunity. Our initial studies revealed that acute ethanol exposure results in transient suppression of phagocytosis of Pseudomonas aeruginosa by macrophages as early as 3 h after initial exposure. Focusing on mechanisms by which ethanol decreases macrophage Fc纬-receptor (Fc纬R) phagocytosis we targeted the study on the focal adhesion and cytoskeletal elements that are necessary for phagosome progression. Ethanol inhibited macrophage phagocytosis of IgG-coated bead recruitment of actin to the site of the phagosome, dampened the phosphorylation of vinculin, but had no effect on paxillin phosphorylation suggesting a loss in 鈥減hagosomal adhesion鈥?maturation. Moreover, our observations revealed that Fc纬R-phagocytosis induced Rac activation, which was increased by only 50%in ethanol exposed cells, compared to 175%in the absence of ethanol. This work is the first to show evidence of the cellular mechanisms involved in the ethanol-induced suppression of Fc纬R-mediated phagocytosis.