ROPGEF1 and ROPGEF4 are functional regulators of ROP11 GTPase in ABA-mediated stomatal closure in Arabidopsis

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• 作者：Zixing Li ; Dong Liu ; ^{liu-d@mail.tsinghua.edu.cn}
• 关键词：GEF ; guanine nucleotide exchange factors ; CA ; constitutively active ; DN ; dominant-negative ; LUC ; luciferase ; LCI ; luciferase complementation imaging ; Y2H ; yeast two-hybrid ; BiFC ; bimolecular fluorescence complementation ; YFP ; yellow fluorescence protein
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：7 May, 2012
• 卷：586
• 期：9
• 页码：1253-1258
• 文件大小：1201 K

摘要

ROPs constitute a family of plant-specific, RHO-like small GTPases that serve as molecular switches in a wide range of signaling pathways. The activities of ROPs are regulated by guanine nucleotide exchange factors (GEFs). ROP11, a member of the ROP GTPase family in Arabidopsis, is a negative regulator of multiple ABA responses. In this study, we show that ROPGEF1 and ROPGEF4 interact with ROP11 on plasma membranes in guard cells. Furthermore, our analyses of ROPGEF1/4 knockout mutants and overexpressing lines suggested that ROPGEF1 and ROPGEF4 are specific regulators of ROP11 function in ABA-mediated stomatal closure.

Structured summary of protein interactions

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