Electrochemical sensor for guanine using a self-assembled monolayer of 1,8,15,22-tetraaminophthalocyanatonickel(II) on glassy carbon electrode

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• 作者：A. John Jeevagan ; S. Abraham John ; ^{abrajohn@yahoo.co.in}
• 关键词：Self-assembled monolayer ; 4伪-Tetraaminophthalocyanatonickel(II) ; Guanine ; Adenine ; Cyclic voltammetry
• 刊名：Analytical Biochemistry
• 出版年：2012
• 期刊代码：93_00032697
• 类别：imm
• 出版时间：1 May, 2012
• 卷：424
• 期：1
• 页码：21-26
• 文件大小：914 K

摘要

This article describes the selective determination of guanine (G) using the self-assembled monolayer (SAM) of 1,8,15,22-tetraaminophthalocyanatonickel(II) (4伪-Ni^IITAPc) modified glassy carbon electrode (GCE) in 0.2 M acetate buffer solution (pH 4.0). The SAM of 4伪-Ni^IITAPc was formed on GCE by spontaneous adsorption of 1 mM 4伪-Ni^IITAPc in dimethylformamide (DMF). It shows two pairs of redox waves corresponding to Ni^III/Ni^II and Ni^IIIPc^-1/Ni^IIIPc^鈭? in 0.2 M acetate buffer solution. The SAM modified electrode exhibits excellent electrocatalytic activity toward the oxidation of G by enhancing its oxidation current with 150 mV less positive potential shift in contrast to bare GCE. Furthermore, the SAM modified electrode selectively determines G in the presence of high concentration of adenine (A). In differential pulse voltammetry measurements, the oxidation current response of G was increased linearly in the concentration range of 10 to 100 渭M, and a detection limit was found to be 3 脳 10^鈭? M (signal/noise = 3).