Transcriptome-based antigen identification for Neisseria meningitidis
- 作者：Kurz ; Sebastian ; Hü ; bner ; Claudia ; Aepinus ; Christian ; Theiss ; Stephanie ; Guckenberger ; Matthias ; et. al.
- 关键词：Meningococci ; Serogroup B ; Antigen screening ; Transcriptome analysis ; DNA microarrays
- 刊名：Vaccine
- 出版年：2003
- 期刊代码：89_0264410x
- 类别：imm
- 出版时间：January 30, 2003
- 卷：21
- 期：7-8
- 页码：768-775
- 文件大小：138 K
摘要
The identification of suitable antigens is crucial to successful vaccine development based on subunit approaches. While many methods exist for the identification of vaccine candidates which are surface-exposed or secreted, immunogenic and conserved, contain B and T cell epitopes, most of these have a major drawback: they do not yield any information on whether the antigen is indeed expressed by the pathogen during infection. However, DNA microarrays offer a novel tool for the investigation of the transcriptional activity of all genes of a pathogenic microorganism under in vivo conditions. Employing whole genome DNA microarrays, we have analyzed the transcriptome of Neisseria meningitidis serogroup B bacteria during different stages of infection, i.e. exposure to human serum and the interaction with human epithelial and endothelial cells. Combined with data derived from genome-based approaches (such as reverse vaccinology) and immunogenicity studies, this novel transcriptome-based antigen identification should reveal ideal vaccine candidates against serogroup B meningococcal infection.