Determination of alachlor and its metabolites in rat plasma and urine by liquid chromatography–electrospray ionization mass spectrometry

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• 作者：Zang ; Lun-Yi ; DeHaven ; Jean ; Yocum ; Aaron ; Qiao ; Guilin
• 刊名：Journal of Chromatography B ; Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2002
• 期刊代码：124_15700232
• 类别：ch
• 出版时间：February 5, 2002
• 卷：767
• 期：1
• 页码：93-101
• 文件大小：265 K

摘要

A method based on liquid chromatography (LC) in combination with mass spectrometry (MS) for the analysis of alachlor (ALA) and its metabolites, 2-chloro-N-[2,6-diethylphenyl]acetamide (CDEPA) and 2,6-diethylaniline (DEA), in rat plasma and urine has been developed. ¹³C-labeled ALA was used as the internal standard for quantitation. The analyte in plasma or urine was isolated using a Waters Oasis HLB extraction plate. The mass spectrometer was operated in the ESI MS-SIM mode with a programming procedure. The retention times for ALA, CDEPA and DEA were 1.84, 3.11 and 4.12 min, respectively. The limits of quantification (LOQ) for ALA, CDEPA and DEA were 2.3, 0.8 and 0.8 ng per injection, respectively. The linear fit of analyte to mass response had an R² of 0.99. Reproducibility of the sample handling and LC–MS analysis had a RSD of ≤10%. The average recoveries for these analytes in rat plasma were better than 90%. Similar results were obtained with rat urine.