Polyamine-functionalized carbon quantum dots (CQDs) with high fluorescence quantum yield (42.5%) have been synthesized by the low temperature (<200 掳C) carbonization of citric acid with branched polyethylenimine (BPEI) in one simple step. The obtained BPEI-CQDs are spherical graphite nanocrystals (average 6.2 nm in size) capped with abundant BPEI at their surfaces. It is the first report that CQDs are both amino-functionalized and highly fluorescent, which suggests their promising applications in chemical sensing.