Antibody levels against B. pertussis in neonates measured in dried blood spots

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• 作者：C.C.N. van Ommen^a ; ^{christivanommen@hotmail.com} ; L.H. Elvers^b ; ^{bert.elvers@rivm.nl} ; D.W. Notermans^b ; ^{daan.notermans@rivm.nl} ; J.C.M. van Huisseling^c ; ^{hans.van.huisseling@ghz.nl} ; P.F.M. Teunis^d ; ^e ; ^{peter.teunis@rivm.nl} ; F.G.A. Versteegh^f ; ^{florens.versteegh@ghz.nl}
• 关键词：Bordetella pertussis ; IgG-PT ; Blood spot ; Guthrie card
• 刊名：Vaccine
• 出版年：2012
• 期刊代码：89_0264410x
• 类别：imm
• 出版时间：30 March, 2012
• 卷：30
• 期：16
• 页码：2697-2700
• 文件大小：312 K

摘要

Aim

We designed this study to investigate if immunoglobuline G-Pertussis toxin (IgG-PT) against Bordetella pertussis in umbilical cord blood can reliably be determined in dried blood spots on filter paper (Guthrie) cards.

Patients and methods

We prospectively included 129 mothers and their newborns born in a general hospital in the Netherlands. The relation between IgG-PT against B. pertussis from the umbilical cord measured in dried blood spots (Guthrie card) and in serum samples was studied by means of a Bland-Altman graph, using regression analysis to evaluate the level of agreement of both measurement methods.

Results

IgG-PT in Guthrie cards show a high coefficient of correlation with IgG-PT in serum samples from the umbilical cord when calibrated against blood spot calibrators (p < 0.05).

Conclusion

Maternal IgG-PT against B. pertussis measured in cord blood applied to Guthrie cards and calibrated against blood spot calibrators show good agreement with measurement of IgG-PT in cord serum. This offers new perspectives for future studies concerning B. pertussis antibodies.