Kinetics of aerobic phenol biodegradation by the acidophilic and hyperthermophilic archaeon Sulfolobus solfataricus 98/2
- 作者：Pierre Christen<sup> ; sup><sup>pierre.christen@univmed.frsup> ; Armando Vega<sup>1sup> ; Laurence Casalot ; Gwenola Simon ; Richard Auria
- 关键词：Phenol ; Biodegradation ; Hyperthermophiles ; Batch culture ; Growth kinetics ; Haldane model
- 刊名：Biochemical Engineering Journal
- 出版年：2012
- 期刊代码：9_1369703x
- 类别：ce
- 出版时间：15 March, 2012
- 卷：62
- 期：Complete
- 页码：56-61
- 文件大小：328 K
摘要
Biodegradation of 51-745 mg l<sup>鈭?sup> of phenol by a well-acclimatized strain of Sulfolobus solfataricus, a thermoacidophilic archaeon, was studied in batch experiments at 80 掳C and pH 3.2. Phenol inhibited growth and specific degradation rates (渭 and q<sub>Ssub>). Fitting the experimental growth data with the Haldane model gave the following kinetic parameters: 渭* = 0.094 h<sup>鈭?sup>, K<sub>Ssub> = 77.7 mg l<sup>鈭?sup>, K<sub>isub> = 319.4 mg l<sup>鈭?sup> (R<sup>2sup> = 0.950). The true 渭<sub>maxsub>, calculated from 渭*, was 0.047 h<sup>鈭?sup>. A volumetric degradation rate (V<sub>maxsub>) was calculated by fitting the phenol consumption data with the Gompertz model. The value of V<sub>maxsub> increased with initial phenol concentration (S<sub>isub>) up to 14.4 mg l<sup>鈭?sup> h<sup>鈭?sup>. The q<sub>Ssub> values, calculated from V<sub>maxsub>, were fitted with the Haldane equation, yielding q<sub>Smaxsub> of 0.110 g g<sup>鈭?sup> h<sup>鈭?sup>. The yield factor (Y<sub>X/Ssub>) depends on S<sub>isub> and reached a maximum of 0.83 g g<sup>鈭?sup> at S<sub>isub> = 93 mg l<sup>鈭?sup>.
S. solfataricus 98/2 displayed low 渭<sub>maxsub> and q<sub>Smaxsub> but a good tolerance to phenol (fairly high K<sub>isub>, , high Y<sub>X/Ssub>). This ability to grow on and degrade phenol (93 mg l<sup>鈭?sup> < optimal S<sub>isub> < 175 mg l<sup>鈭?sup>) at high temperature and low pH is unique and may be useful for removing phenol from hot acidic contaminated effluents. Other possible application could lie in the production of the enzymes involved in the key steps of phenol degradation provided the cloning of the enzymes-related genes in fast-growing mesophiles.