Protective efficacy of an H1N1 cold-adapted live vaccine against the 2009 pandemic H1N1, seasonal H1N1, and H5N1 influenza viruses in mice

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• 作者：Jianzhong Shi^a ; Zhiyuan Wen^a ; Jing Guo^a ; Ying Zhang^a ; Guohua Deng^a ; Yuelong Shu^b ; Dayan Wang^b ; Yongping Jiang^a ; Yoshihiro Kawaoka^c ; Zhigao Bu^a ; Hualan Chen^a ; ^{hlchen1@yahoo.com}
• 关键词：Influenza ; Live attenuated vaccine ; H1N1 ; H5N1
• 刊名：Antiviral Research
• 出版年：2012
• 期刊代码：7_01663542
• 类别：imm
• 出版时间：March, 2012
• 卷：93
• 期：3
• 页码：346-353
• 文件大小：830 K

摘要

Vaccination is a key strategy for preventing influenza virus infections. Here, we generated a reassortant virus (SC/AAca) containing the hemagglutinin and neuraminidase genes from a 2009 pandemic influenza virus A/Sichuan/1/2009 (H1N1) (SC/09) and six internal genes from the cold-adapted virus A/Ann Arbor/6/60 (H2N2) (AAca). The SC/AAca reassortant induced a sound humoral immune response and complete protection against homologous SC/09 virus challenge in mice after intranasal administration of an at least 10⁶ 50%egg infectious dose (EID₅₀) of SC/AAca. SC/AAca inoculation also induced significant CD4+ and CD8+ T cell responses and provided solid protection against heterologous H1N1 and H5N1 virus challenge. Our results suggest that this 2009 H1N1 live vaccine will provide protection against both 2009 pandemic and seasonal H1N1 virus infection and might reduce the severity of H5N1 virus infection in humans. The induction of cross-reactive virus-specific T cell responses may be an effective approach to develop universal influenza vaccines.