HPLC and chemometrics-assisted UV-spectroscopy methods for the simultaneous determination of ambroxol and doxycycline in capsule

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• 作者：Ghada M. Hadad ; Alaa El-Gindy ; Waleed M.M. Mahmoud
• 关键词：Ambroxol ; Doxycycline ; HPLC ; Multivariate spectrophotometric methods
• 刊名：Spectrochimica Acta Part A ; Molecular and Biomolecular Spectroscopy
• 出版年：2008
• 期刊代码：99_13861425
• 类别：ch
• 出版时间：August 2008
• 卷：70
• 期：3
• 页码：655-663
• 文件大小：193 K

摘要

High-performance liquid chromatography (HPLC) and multivariate spectrophotometric methods are described for the simultaneous determination of ambroxol hydrochloride (AM) and doxycycline (DX) in combined pharmaceutical capsules. The chromatographic separation was achieved on reversed-phase Cb>18b> analytical column with a mobile phase consisting of a mixture of 20 mM potassium dihydrogen phosphate, pH 6–acetonitrile in ratio of (1:1, v/v) and UV detection at 245 nm. Also, the resolution has been accomplished by using numerical spectrophotometric methods as classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS-1) applied to the UV spectra of the mixture and graphical spectrophotometric method as first derivative of the ratio spectra (¹DD) method. Analytical figures of merit (FOM), such as sensitivity, selectivity, analytical sensitivity, limit of quantitation and limit of detection were determined for CLS, PLS-1 and PCR methods. The proposed methods were validated and successfully applied for the analysis of pharmaceutical formulation and laboratory-prepared mixtures containing the two component combination.