Development of dual gradient column in liquid chromatography
- 作者：Toyoharu Oda ; Shinya Kitagawa and Hajime Ohtani
- 关键词：Dual gradient ; Flow velocity ; Chemical property ; Liquid chromatography ; Retention behavior
- 刊名：Journal of Chromatography A
- 出版年：2006
- 期刊代码：47_00219673
- 类别：ch
- 出版时间：10 February 2006
- 卷：1105
- 期：1-2
- 页码：154-158
- 文件大小：127 K
摘要
The dual gradient column, in which both the chemical property of the stationary phase and the flow velocity in the mobile phase are heterogeneous longitudinally along the column, is developed to obtain the mobile phase gradient-like elution in an isocratic condition. Here, the step-wise dual gradient columns were prepared by connecting an inlet column (I.D. 50 μm, packed with ODS) serially to an outlet column (I.D. 100–200 μm, packed with the mixture of ODS and C1 [9:1]). The retention behavior of alkylbenzenes was able to be controlled in the dual gradient column depending on the variation in the flow velocity. Moreover, the change in retention behavior induced by the flow velocity variation for the dual gradient columns was quite different from that by the variation in organic modifier content of the mobile phase in isocratic elution for a single gradient column and can induce the similar effect with an ordinary gradient elution in a mobile phase composition.