Micropropagation of Pistacia lentiscus L. from axenic seedling-derived explants

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• 作者：Hakan Y谋ld谋r谋m ; ^{hakanyildirim75@hotmail.com}
• 关键词：BA ; N6-benzyladenine ; DKW ; Driver and Kuniyuki ; IBA ; indole butyric acid ; IAA ; indole-3-asetic acid ; KIN ; kinetin ; MS ; Murashige and Skoog medium ; PGR ; plant growth regulators ; PVP ; polyvinyl pyrollidone ; QL ; Quoirin and Lepoivre ; WPM ; woody plant medium
• 刊名：Scientia Horticulturae
• 出版年：2012
• 期刊代码：93_03044238
• 类别：abs
• 出版时间：1 April, 2012
• 卷：137
• 期：Complete
• 页码：29-35
• 文件大小：887 K

摘要

Pistacia lentiscus L. (Anacardiaceae), a deciduous forest tree, is important multipurpose Pistacia tree. Today, the major limitation facing the widespread expansion of commercial lentisk plantations is the shortage of superior plants primarily because of difficulties experienced in propagating this species by using the traditional vegetative propagation methods. Shoot cultures were established from aseptically germinated seedlings of lentisk. Factors including the different N⁶-benzyladenine (BA) concentrations, the combination of cytokinin and other growth regulators, media and antioxidants were assessed and optimized for in vitro shoot proliferation. Full strength MS medium with Gamborg's vitamins containing 30 g l^鈭? sucrose, 100 mg l^鈭? PVP, 1 mg l^鈭? BA and 7 g l^鈭? agar resulted in multiple shoot (bud) initiation at the rate of 2.7 卤 0.17 shoot (4.18 卤 0.17 bud) per explant in 28 days of culture. Moreover, with the use of in vitro proliferated axenic cultures subcultured at least twice, the effects of auxins and mineral medium strength were also assessed for root induction. Efficient rooting (92%) was achieved in a medium containing 1 mg l^鈭? indole-3-butyric acid (IBA). The method developed for plant acclimatization was satisfactory because a high percentage of plant survival (83.33%) in the growth room was obtained and the regenerated plantlets resumed growth after 4 months (96%). The method described will be useful for rapid multiplication of lentisk for commercial exploitation.