A functional polymorphism in the parkin gene promoter affects the age of onset of Parkinson's disease
- 作者：Greg Sutherl ; George Mellick ; Carolyn Sue ; Daniel K.Y. Chan ; Dominic Rowe ; Peter Silburn ; Glenda Halliday
- 关键词：Genotype ; Parkin ; Parkinson's disease ; Onset
- 刊名：Neuroscience Letters
- 出版年：2007
- 期刊代码：52_03043940
- 类别：neu
- 出版时间：6 March 2007
- 卷：414
- 期：2
- 页码：170-173
- 文件大小：145 K
摘要
Mutations in the parkin gene are the major cause of autosomal recessive early-onset forms of Parkinson's disease (PD). As reduced parkin expression might also affect the clinical course of idiopathic PD we investigated the effect of a low expressing allele in the parkin promoter region on the age at disease onset (AAO). Patients with PD (n = 175) fulfilling standard diagnostic criteria were recruited by experienced neurologists at two movement disorders clinics in Sydney and Brisbane, Australia. DNA was extracted from whole blood and the −258 T/G polymorphism genotyped using PCR/RFLP. AAO effects were analysed using univariate ANOVA, binomial logistic regression modelling and Kaplan-Meier survival analysis. Subjects with the GG genotype (n = 10, mean AAO = 46.2 ± 11.5 (S.D.) years) had a significantly lower mean AAO compared to the common TT genotype (n = 104, mean AAO = 56.1 ± 12.7, p = 0.02). There was no difference in mean AAO between the TT and TG individuals (n = 61, mean AAO = 55.3 ± 11.6). Stratifying the sample by median AAO (55 years) revealed that the GG genotype was over-represented in the early-onset group (n = 9, OR = 18.6, 95%CI = 1.41–245.3, p = 0.03). We speculate that reduced expression of normal parkin protein may result in an early manifestation of PD symptoms.