Evaluation of collagen alteration after topical photodynamic therapy (PDT) using second harmonic generation (SHG) microscopy - in vivo study in a mouse model

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• 作者：Ting Lv^a ; ¹ ; Zu-Fang Huang^b ; ¹ ; Hong-Wei Wang^a ; Ju-Qiang Lin^b ; Guan-Nan Chen^b ; Xi-Wen Chen^b ; Rong Chen^b ; Zheng Huang^b ; ^c ; ^d ; Xiu-Li Wang ; PhD ; MD^a ; ^{xlwang2001@yahoo.cn}
• 关键词：Photodynamic therapy ; Second harmonic generation ; 5-ALA ; Collagen ; Skin rejuvenation
• 刊名：Photodiagnosis and Photodynamic Therapy
• 出版年：2012
• 期刊代码：255_15721000
• 类别：med
• 出版时间：June, 2012
• 卷：9
• 期：2
• 页码：164-169
• 文件大小：1571 K

摘要

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Summary

Topical photodynamic therapy (PDT) mediated by 5-aminolevulinic acid (ALA) has been used for the treatment of age-related skin lesions for therapeutic or cosmetic purposes. The modulation of collagen component and structure might play a significant role in influencing treatment outcomes of PDT. In this study, the effect of multi-session low dose ALA PDT on skin rejuvenation was examined in a hairless mouse model. Changes in collagen and skin texture were investigated by histological examination and in vivo second harmonic generation (SHG) microscopy. Results indicated that multi-session PDT could improve the collagen density and arrangement of skin tissue. SHG microscopy combined with quantitative collagen analysis could provide a useful tool for the evaluation of collagen alteration induced by ALA PDT.