Matrix-assisted laser desorption/ionization mass spectrometry (MALDI TOF MS) study of Huperzine A, a natural anti-Alzheimer's disease product, its derivatization and its detection by highly sensitive
- 作者：A. Ben Hameda ; P. Tá ; borský ; E.M. Peñ ; a-Mé ; ndez ; J. Havel
- 关键词：Huperzine A ; Derivatization ; MALDI TOF MS ; CE-LIF
- 刊名：Talanta
- 出版年：2007
- 期刊代码：106_00399140
- 类别：ch
- 出版时间：30 April 2007
- 卷：72
- 期：2
- 页码：780-784
- 文件大小：271 K
摘要
Huperzine A, a reversible acetylcholinesterase inhibitor for the treatment of Alzheimer disease (HupA), was studied using an (MALDI TOF MS) instrument in MALDI mode. The formation of a HupA dimmer in a vacuum was observed and several matrices were found that were able to inhibit its formation. The structures of the neutral and protonated form of the HupA molecule were calculated and optimized using a Hyperchem program. Detection limit using MALDI TOF MS in the model sample was 5.3 pg. MALDI TOF MS was also applied to the direct detection of the drug in medical preparations and in human serum. The limit of detection in plasma was 14.2 pg with a signal-to-noise ratio of 3:1. However, the sensitivity was not as high as it usually is in MALDI. Therefore, a new method for the derivatization of HupA was developed using fluorescent labelling with rhodamine B isothiocyanate (RBITC). A limit of detection using capillary electrophoresis laser induced fluorescence detection (CE-LIF) equal to 4 × 10p>−9 p> mol lp>−1 p> was reached.