Biotinidase determination in serum and dried blood spots—high sensitivity fluorimetric ultramicro-assay
- 作者：Broda ; Elke ; Baumgartner ; E. Regula ; Scholl ; Sabine ; Stopsack ; Marina ; Horn ; Anton ; Rhode ; Heidrun
- 关键词：Biotinidase deficiency ; Screening ; Fluorimetry ; AQ ; 6-amidoquinoline ; BAQ ; biotin 6-amidoquinoline ; MES ; Morpholinoethansulfonic acid ; Tris ; Tris(hydroxymethyl)aminomethan ; MOPS ; 3-(N-morpholino)propansulfonic acid ; HEPES ; N-2-hydroxyethylpiperazin-N&prim
- 刊名：Clinica Chimica Acta
- 出版年：2001
- 期刊代码：54_00098981
- 类别：med
- 出版时间：December, 2001
- 卷：314
- 期：1-2
- 页码：175-185
- 文件大小：139 K
摘要
A miniaturized quantitative biotinidase assay has been developed using biotin 6-amidoquinoline as substrate and the 100-fold enhanced fluorescence of 6-amidoquinoline measured using apolar solvents. Amidoquinoline is measured after deproteinization by ethanol/acetone using individual standardisation and solvent resistant microtiter plates. The assay was optimized for end point determinations of biotinidase activities in serum and for newborn screening using dried blood spots.