Escape mutants of pandemic influenza A/H1N1 2009 virus: Variations in antigenic specificity and receptor affinity of the hemagglutinin

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• 作者：Irina Rudneva^a ; ^{irinarydneva@gmail.com} ; Anna Ignatieva^a ; Tatiana Timofeeva^a ; Aleksandr Shilov^a ; Alla Kushch^a ; Olga Masalova^a ; Regina Klimova^a ; Nicolai Bovin^b ; Larisa Mochalova^c ; Nikolai Kaverin^a
• 关键词：HA ; hemagglutinin ; NA ; neuraminidase ; MAb ; monoclonal antibody ; HI ; hemagglutination inhibition ; ELISA ; enzyme-linked immunosorbent assay ; PCR ; polymerase chain reaction ; PAA ; polyacrylamide ; RBC ; red blood cells ; 3&prime ; SL ; Neu5Ac伪2-3Gal尾1-4Glc尾 ; 3&p
• 刊名：Virus Research
• 出版年：2012
• 期刊代码：92_01681702
• 类别：imm
• 出版时间：June, 2012
• 卷：166
• 期：1-2
• 页码：61-67
• 文件大小：411 K

摘要

A panel of 6 neutralizing monoclonal antibodies (MAbs) raised against A/Moscow/IIV01/2009 (H1N1) virus isolated during the 2009 pandemic was used for the selection of 26 escape mutants. The mutants were characterized in immune cross-reactions with the panel of MAbs. The sequencing of the mutant HA genes revealed 5 amino acid positions recognized by monoclonal antibodies: 129, 156, 158, 159, and 190 (H3 numbering). The amino acid positions were distributed in two epitopes belonging to antigenic sites Sa and Sb. The mutant HAs exhibited variations in the affinity to synthetic high molecular mass sialic acid-containing receptor analogues. Results are discussed in connection with the antigenic drift potential of the 鈥渟wine-like鈥?pandemic 2009 influenza virus.