Expression of Kaposi's sarcoma-associated herpesvirus-encoded K10/10.1 protein in tissues and its interaction with poly(A)-binding protein
- 作者：Takayuki Kanno ; Yuko Sato ; Tetsutaro Sata and Harutaka Katano
- 关键词：Kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) ; K10/10.1 ; Kaposi's sarcoma (KS) ; Multicentric Castleman's disease (MCD) ; Poly(A)-binding protein (PABP) ; Primary effusion lymphoma (PEL)
- 刊名：Virology
- 出版年：2006
- 期刊代码：108_00426822
- 类别：imm
- 出版时间：15 August 2006
- 卷：352
- 期：1
- 页码：100-109
- 文件大小：728 K
摘要
The K10/10.1 protein is encoded by a cluster of interferon regulatory factor (IRF) homologues in the Kaposi's sarcoma-associated herpesvirus (KSHV, human herpesvirus 8, HHV-8) genome. In the present study, we showed that an anti-K10 antibody reacted with a 110-kDa protein encoded by the K10/10.1 gene of KSHV in KSHV-infected primary effusion lymphoma (PEL) cell lines. Expression of K10/10.1 protein was induced by phorbol ester in KSHV-infected cells. A reporter gene assay demonstrated that K10/10.1 protein did not influence promoter activity of human interferon genes, regardless of its homology to human IRFs. Poly(A)-binding protein (PABP) was identified as a partner of K10/10.1 protein. Immunoprecipitation revealed that K10/10.1 protein interacted with PABP specifically in PEL cell lines. IFA revealed co-localization of K10/10.1 protein and PABP in the nucleus of KSHV-infected cells. These data suggest that K10/10.1 protein may affect the translational status or stability of mRNA in host cells.