• 作者：J.J. Echevarria Uraga^a ; ^{FIZER@telefonica.net} ; I. Garcí ; a-Alonso Montoya^b ; I. Dí ; az Sanz^b ; B. Herrero de la Parte^b ; J.L. Migué ; lez Vidales^a ; I. Zabalza Esté ; vez^c ; B. Ferná ; ndez-Ruanova^d
• 关键词：Neoplasia ; Neoplasia del hí ; gado ; experimental ; Neoplasia ; experimental ; Ecografí ; a ; Experimentació ; n animal
• 刊名：Radiolog铆a
• 出版年：2010
• 期刊代码：pca298_00338338
• 类别：med
• 出版时间：January-February 2010
• 卷：52
• 期：1
• 页码：37-44
• 文件大小：373 K

Objective

To determine the ultrasonographic characteristics of liver metastases induced in a rat model; to evaluate the usefulness of ultrasonography in the noninvasive evaluation of tumor infiltration.

Material and methods

We seeded the livers of 46 WAG/RijCrl rats with CC-531 syngeneic colorectal carcinoma cells by intrasplenic inoculation. At 21, 28, 35, 42, 70 days after tumor seeding, we performed a series of ultrasonographic examinations to evaluate tumor induction in different groups of animals: 37 rats were studied with a 10 MHz linear probe and 9 were studied with a 6鈥?8 MHz multifrequency probe. The following signs were considered indicative of tumor development: intrahepatic nodules, sinuate liver borders, lobe enlargement, and extrahepatic masses. Ultrasonographic findings were verified at autopsy. We determined the number of implants, size (less than 3 mm, between 3 and 7 mm, or greater than 7 mm), and lobe location for each technique.

Results

Compared to the autopsy results, ultrasonography detected 64%of the animals with disease. All the extrahepatic masses were correctly diagnosed. Metastases were identified in 90%of the rats with lesions greater than 7 mm, in 75%of those with implants between 3 and 7 mm, and in 25%of those with lesions less than 3 mm. In the group in which we used the 6鈥?8 MHz probe, we detected 50%of the lesions less than 3 mm.

Conclusion

Ultrasonography was useful for monitoring the experimental model and enabled the noninvasive oncologic evaluation of the rat liver with reasonable sensitivity.