Wolbachia endosymbiont levels in severe and mild strains of Onchocerca volvulus
- 作者：Higazi ; Tarig B. ; Filiano ; Anthony ; Katholi ; Charles R. ; Dadzie ; Yankum ; Remme ; Jan H. ; Unnasch ; Thomas R.
- 关键词：Filariasis ; River blindness ; Wolbachia
- 刊名：Molecular & Biochemical Parasitology
- 出版年：2005
- 期刊代码：108_01666851
- 类别：bio
- 出版时间：May, 2005
- 卷：141
- 期：1
- 页码：109-112
- 文件大小：132 K
摘要
Epidemiological, clinical and genetic data have all suggested that the filarial parasite Onchocerca volvulus, the causative agent of onchocerciasis (or river blindness) exists as two strains in West Africa. The severe strain induces severe ocular disease in a large proportion of the infected population, while the mild strain induces little ocular disease. Although DNA probes based upon a non-coding repeat sequence family can distinguish the two strains, the underlying basis for this difference in pathogenicity is not understood. Recently, several studies have implicated products produced by the Wolbachia endosymbiotic bacterium of O. volvulus in the pathogenesis of onchocerciasis. This suggested the hypothesis that differences in the Wolbachia endosymbiont population might be responsible for the pathogenic differences noted in the two strains. To test this hypothesis, quantitative PCR assays were used to measure the amount of Wolbachia DNA per nuclear genome in a collection of well characterized samples of mild and severe strain O. volvulus. The median ratio of Wolbachia DNA to nuclear DNA was significantly greater in severe strain parasites than in mild strain parasites. These data support the hypothesis that the pathogenic differences seen in severe and mild strain O. volvulus may be a function of their relative Wolbachia burden and provide additional support to the hypothesis that Wolbachia products may play a central role in the pathogenesis of ocular onchocerciasis.