P₄-ATPase ATP8A2 acts in synergy with CDC50A to enhance neurite outgrowth

详细信息	查看全文 | 推荐本文 |

• 作者：Qin Xu^a ; Guo-Ying Yang^b ; Na Liu^a ; Peng Xu^a ; Yue-Lei Chen^a ; Zheng Zhou^b ; Zhen-Ge Luo^b ; Xiaoyan Ding^a ; ^{xyding@sibs.ac.cn} ; ^{xyding@sunm.shcnc.ac.cn}
• 关键词：P4-ATPase ; CDC50 protein ; Neuronal differentiation ; Flippase
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：21 June, 2012
• 卷：586
• 期：13
• 页码：1803-1812
• 文件大小：1457 K

摘要

P₄-ATPases are lipid flippases that transport phospholipids across cellular membranes, playing vital roles in cell function. In humans, the disruption of the P₄-ATPase ATP8A2 gene causes a severe neurological phenotype. Here, we found that Atp8a2 mRNA was highly expressed in PC12 cells, hippocampal neurons and the brain. Overexpression of ATP8A2 increased the length of neurite outgrowth in NGF-induced PC12 cells and in primary cultures of rat hippocampal neurons. Inducing the loss of function of CDC50A in hippocampal neurons via RNA interference reduced neurite outgrowth, and the co-overexpression of CDC50A and ATP8A2 in PC12 cells enhanced NGF-induced neurite outgrowth. These results indicate that ATP8A2, acting in synergy with CDC50A, performs an important role in neurite outgrowth in neurons.