Disruption of the Plasmodium berghei 2-Cys peroxiredoxin TPx-1 gene hinders the sporozoite development in the vector mosquito
- 作者：Kazuhiko Yano ; Hitoshi Otsuki ; Meiji Arai ; Kanako Komaki-Yasuda ; Takafumi Tsuboi ; Motomi Torii ; Shigeyuki Kano ; Shin-Ichiro Kawazu
- 关键词：Insect stage ; Peroxiredoxin ; Plasmodium berghei ; Thioredoxin peroxidase
- 刊名：Molecular and Biochemical Parasitology
- 出版年：2008
- 期刊代码：108_01666851
- 类别：bio
- 出版时间：June 2008
- 卷：159
- 期：2
- 页码：142-145
- 文件大小：417 K
摘要
To investigate the physiologic role of cytosolic 2-Cys peroxiredoxin of Plasmodium berghei (PbTPx-1), we infected the vector mosquito Anopheles stephensi with a parasite carrying a targeted knockout of pbtpx-1 (Prx-KO). The number of Prx-KO midgut oocysts at 14–15 days post-feeding (pf) was comparable to that of the parent strain (WT); however, the numbers of sporozoites that formed in midgut oocysts and accumulated in the salivary gland of Prx-KO-infected mosquitoes by 21 days pf were decreased to 10–20%and 3–10%, respectively, of those values in WT-infected mosquitoes. A higher frequency of DNA strand breaks was detected in Prx-KO oocysts than in WT oocysts. Sporozoites carrying the targeted disruption had reduced infectivity in mice; however, the knockout did not affect the ability of the sporozoite to reach the liver parenchyma and initiate exo-erythrocytic form (EEF) development. TPx-1 may be involved in development during exponentially multiplying stages, such as sporozoites and EEF.