In this study, time-averaged gas holdup distributions were investigated in a 16 cm diameter bubble column for two-phase dispersed system of air–water and air–glycerol solution of 10 wt%by using ultrasonic computed tomography (UCT). A quantitative result of UCT – as a coupling of the ultrasonic transmission method and the iterative filtered backprojection (IFBP) image reconstruction technique – is presented. The UCT results are in a good agreement with those by the bed expansion method. A higher gas holdup in the air–glycerol 10 wt%solution than in the air–water system was observed. The distribution of gas holdup in the column with an attached baffle is also investigated by UCT.