DNA template-assisted modulation of horseradish peroxidase activity
- 作者：Shohini Ghosh Datta ; Xiaozheng Dou ; Alyxandria Shibley ; Bhaskar Datta ; BhaskarDatta@MissouriState.edu
- 关键词：DNA-templated synthesis ; Horseradish peroxidase ; Polyphenols ; Enzymatic oxidation ; Oxidation of phenolic derivatives
- 刊名：International Journal of Biological Macromolecules
- 出版年：2012
- 期刊代码：62_01418130
- 类别：abs
- 出版时间：1 April, 2012
- 卷：50
- 期：3
- 页码：552-557
- 文件大小：1081 K
摘要
The precise alignment of DNA molecules by Watson-Crick base-pairing combined with its polymeric characteristics have allowed DNA to be used as a template or scaffold for assembling materials. In this work, we investigate the role of calf-thymus DNA as a template for enhancing the horseradish peroxidase (HRP)-mediated oxidation of phenol and phenolic derivatives. The HRP-catalyzed oxidation of phenol into polyphenolic products and in presence of 4-aminoantipyrine into quinoneimine dye complexes is studied. Visible spectroscopy reveals an increased yield of both products of the enzymatic reaction in presence of calf-thymus DNA and is attributed to the prearrangement of the corresponding substrates on the DNA. The concentrations of calf-thymus DNA and the substrates are found to affect the nature of prearrangement and subsequent formation of polymeric or co-oxidation products. Also, phenolic derivatives with different aromatic substitutions display divergent propensities towards product formation in presence of the DNA template. Our results demonstrate the ability of calf-thymus DNA to modulate the activity of HRP and exercise control on the nature of products formed. This work highlights the potential of using DNA as a template for influencing enzymatic reactions involving aromatic substrates.