The simultaneous determination of vitamins A, E and 尾-carotene in bovine milk by high performance liquid chromatography-ion trap mass spectrometry (HPLC-MSⁿ)

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• 作者：Tim Plozza^a ; ^{tim.plozza@dpi.vic.gov.au} ; V. Craige Trenerry^a ; Domenico Caridi^b
• 关键词：Quantification ; Vitamin A ; Vitamin E ; 尾-Carotene ; Bovine milk ; Liquid chromatography&ndash ; ion trap mass spectrometry (HPLC&ndash ; MSn)
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：1 September, 2012
• 卷：134
• 期：1
• 页码：559-563
• 文件大小：227 K

摘要

Bovine milk is considered to be a good dietary source of vitamins A, E and 尾-carotene. This paper describes a robust method for the simultaneous determination of these compounds using high performance liquid chromatography-ion trap mass spectrometry (HPLC-MSⁿ) as the determinative step. The compounds were separated with a 5 渭m Polaris 2.1 脳 150 mm C18-A column and an aqueous-methanol mobile phase and the levels determined using the MS² fragments (vitamin A, m/z 213 and m/z 199, vitamin E m/z 165 and 尾-carotene m/z 413). The HPLC-MSⁿ was operated in the positive ion APCI mode. The method was validated using repeatability studies, duplicate analyses, recovery experiments, proficiency study data and comparison with previously validated high performance liquid chromatographic ultraviolet/visible (HPLC-UV/Vis) and fluorescence (HPLC-Fl) procedures used in our laboratory. Expanded measurement uncertainties were similar for HPLC-MSⁿ, HPLC-UV/Vis and HPLC-Fl, e.g. vitamin A 45 渭g/100 ml, HPLC-MSⁿ 卤8%, HPLC-UV 卤9%; vitamin E 150 渭g/100 ml, HPLC-MSⁿ 卤11%, HPLC-Fl 卤9%; 尾-carotene 12 渭g/100 ml, HPLC-MSⁿ 卤18%, HPLC-Vis 卤21%.