Association of hsp70-2 (+ 1267A/G), hsp70-hom (+ 2437T/C), HMOX-1 (number of GT repeats) and TNF-alpha (+ 489G/A) polymorphisms with COPD in Croatian population

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• 作者：Mirela Matokanovi膰^a ; ^{mmatokanovic@pharma.hr} ; Lada Rumora^a ; ^{lrumora@pharma.hr} ; Sanja Popovi膰-Grle^b ; ^{sanja.grle@kbc-zagreb.hr} ; Ivana 膶epelak^a ; ^{icepelak@pharma.hr} ; Ognjen 膶uli膰^c ; ^{ognjenculic@hotmail.com} ; Karmela Bari&scaron ; i膰^a ; ^{kbarisic@pharma.hr}
• 关键词：CI ; confidence interval ; COPD ; chronic obstructive pulmonary disease ; FEV1 ; forced expiratory volume in 1 second ; FVC ; forced vital capacity ; GOLD ; Global Initiative for Chronic Obstructive Lung Disease ; HMOX 1 ; heme oxygenase 1 gene ; hsp70 2 ; heat shock
• 刊名：Clinical Biochemistry
• 出版年：2012
• 期刊代码：55_00099120
• 类别：med
• 出版时间：July, 2012
• 卷：45
• 期：10-11
• 页码：770-774
• 文件大小：167 K

摘要

Objective

To test for possible association of hsp70-2 (+ 1267A/G), hsp70-hom (+ 2437T/C), HMOX-1 (number of GT repeats) and TNF-伪 (+ 489G/A) polymorphisms with chronic obstructive pulmonary disease (COPD) in Croatian population.

Methods

Genotyping of DNA isolated from whole blood of 130 COPD patients (as defined by spirometry) and 95 healthy controls was performed. Fragment size analysis upon restriction enzyme digestion and/or sequencing was used for genotype/allele definition. Significance of findings was tested using 蠂² test.

Results

hsp70-2 (+ 1267A/G) polymorphism was significantly associated with COPD. Results of genotyping analysis indicated that a genotype carrying G allele was preferentially associated with COPD; odds ratio (OR) = 1.50, 95%confidence interval (CI) = 1.00-2.24 and P = 0.061. OR for the GG genotype was 3.47 with CI = 1.26-9.56 and P = 0.04. No association for hsp70-hom (+ 2437T/C), TNF-伪 (+ 489G/A) and HMOX-1 (number of GT repeats) polymorphisms were found. In addition, comparison of genotype frequencies among different stages of disease severity (GOLD II-IV) revealed no discrimination for any of the tested polymorphisms.

Conclusion

This study is supporting the association of hsp70-2 (+ 1267A/G) polymorphism and COPD. Higher frequency of G allele and GG genotype in Croatian COPD patients was observed. There was no evidence for the association of hsp70-hom (+ 2437T/C), TNF-伪 (+ 489G/A) SNPs and HMOX-1 (number of GT repeats) polymorphism with COPD. Allele and genotype frequencies for all of the tested polymorphisms show no association with disease severity (GOLD II-IV).