The concentration-dependent chemokine release and pro-apoptotic effects of neutrophil-derived α-defensin-1 on human bronchial and alveolar epithelial cells
- 作者：Chien-Ying Liu ; Horng-Chyuan Lin ; Chih-Teng Yu ; Shu-Min Lin ; Kang-Yun Lee ; Hao-Chen Chen ; Chun-Liang Chou ; Chien-Da Huang ; Pai-Chien Chou ; Wen-Te Liu ; et al.
- 关键词：Defensin ; Interleukin (IL)-8 ; Monocyte chemoattractant protein (MCP)-1 ; Apoptosis ; Caspase ; Endoplasmic reticulum (ER)
- 刊名：Life Sciences
- 出版年：2007
- 期刊代码：62_00243205
- 类别：imm
- 出版时间：30 January 2007
- 卷：80
- 期：8
- 页码：749-758
- 文件大小：1426 K
摘要
Defensins play a pivotal role in antimicrobial reactions, inflammatory responses, wound repair, and specific immunity. In inflammatory and infectious lung diseases, α-defensins are released from recruited neutrophils, and modulate a variety of lung cell functions. We found that human bronchial and alveolar epithelial cells treated with low and moderate concentrations (5 and 10 μg/ml) of purified neutrophil-derived α-defensin-1 secreted more interleukin (IL)-8 and monocyte chemoattractant protein (MCP)-1 in a dose- and time-dependent manner. Under moderate and high concentrations (10 and 20 μg/ml) of α-defensin-1, we observed typical apoptotic changes in the lung epithelial cells after stimulation for 24 h. Furthermore, α-defensin-1 triggered lung cell detachment in a time- and dose-dependent manner at moderate and high concentrations. Prior to the detachment, caspase-3 activity significantly increased. On confocal laser microscopy, rapid translocation of α-defensin-1 to the endoplasmic reticulum (ER) was noted. These findings suggest that neutrophil-derived α-defensin-1 has pro-inflammatory and apoptotic effects in human bronchial and alveolar epithelial cells, which are concentration-dependent and may be associated with ER activity.