Expression and characterization of two STAT isoforms from Sf9 cells
- 作者：Maw-Sheng Yeh ; Chia-Hsiung Cheng ; Chih-Ming Chou ; Ya-Li Hsu ; Cheng-Ying Chu ; Gen-Der Chen ; Shui-Tsung Chen ; Guang-Chao Chen ; Chang-Jen Huang
- 关键词：STAT ; DNA binding ; Baculovirus ; Insect Sf9 cells
- 刊名：Developmental and Comparative Immunology
- 出版年：2008
- 期刊代码：35_0145305x
- 类别：abs
- 出版时间：2008
- 卷：32
- 期：7
- 页码：814-824
- 文件大小：860 K
摘要
In invertebrates, the JAK–STAT signaling pathway is involved in the anti-bacterial response and is part of an anti-viral response in Drosophila. In this study, we show that two STAT transcripts are generated by alternative splicing and encode two isoforms of Sf-STAT with different C-terminal ends. These two isoforms were produced and purified using the recombinant baculovirus technology. Both purified isoforms showed similar DNA-binding activity and displayed weak but significant transactivation potential toward a Drosophila promoter that contained a STAT-binding motif. No significant activation of the Sf-STAT protein in Sf9 cells was found by infection with baculovirus AcMNPV.