Development of recombinant canine adenovirus type-2 expressing the Gn glycoprotein of Seoul virus

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• 作者：Ziguo Yuan ; Xiuxiang Zhang ; Shoufeng Zhang ; Ye Liu ; Shengyan Gao ; Fei Zhang ; Huijuan Xu ; Xiaohu Wang ; Rongliang Hu
• 关键词：Hantavirus ; Seoul virus ; Canine adenovirus type-2 ; Gn
• 刊名：Biologicals
• 出版年：2008
• 期刊代码：162_10451056
• 类别：bio
• 出版时间：May 2008
• 卷：36
• 期：3
• 页码：162-167
• 文件大小：522 K

摘要

Seoul virus glycoprotein Gn is a major structural protein and candidate antigen of hantavirus that induces a highly immunogenic response for hantavirus vaccine. In this study, a replication-competent recombinant canine adenovirus type-2 expressing Gn was constructed by the in vitro ligation method. The Gn expression cassette, including the human cytomegalovirus (hCMV) promoter/enhancer and the SV40 early mRNA polyadenylation signal, was cloned into the SspI site of the E3 region which is not essential for proliferation of CAV-2. Expression of Gn was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting.