Validation of antibiotics in catfish by on-line solid phase extraction coupled to liquid chromatography tandem mass spectrometry

详细信息	查看全文 | 推荐本文 |

• 作者：Jorge Hurtado de Mendoza^a ; ¹ ; Luana Maggi^c ; ¹ ; Liliana Bonetto^a ; Beatriz Rodrí ; guez Carmena^b ; Alicia Lezana^b ; Francisco A. Mocholí ; ^a ; Manuel Carmona^c ; ^d ; ^{Manuel.Carmona@uclm.es}
• 关键词：Antibiotics ; Residues ; Catfish ; XLC&ndash ; MS/MS
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：15 September, 2012
• 卷：134
• 期：2
• 页码：1149-1155
• 文件大小：514 K

摘要

For the first time automated on-line solid phase extraction coupled to liquid chromatography tandem mass spectrometry was developed for the simultaneous determination of 13 antibiotics (sulfonamides and tetracyclines) in catfish. The method proposed was validated according to , showing good linearity between 2 and 350 渭g kg^鈭?, high recovery (80-99%) and reproducibility (13-20%) values, lower detection limits than 0.1 渭g kg^鈭?, and quantification limits under 2.4 渭g kg^鈭? (between 39 and 84 times lower than the MRL fixed by the EU). Moreover, the proposed method was also used to determine sulfonamides and tetracyclines in 16 out of 107 samples, all previously analysed by microbiological screening that gave positive results. Five out of 13 antibiotics were found, having tetracycline the higher occurrence (10 samples); in all cases the concentrations were lower than the MRL established.