HPLC separation of naringin, neohesperidin and their C-2 epimers in commercial samples and herbal medicines
- 作者：Nahoko Uchiyama ; Ik Hwi Kim ; Ruri Kikura-Hanajiri ; Nobuo Kawahara ; Tenji Konishi ; Yukihiro Goda
- 关键词：Naringin ; Neohesperidin ; Diastereomeric separation ; Chiral stationary phase ; Citrus species ; Kampo formulations
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2008
- 期刊代码：65_07317085
- 类别：ch
- 出版时间：14 April 2008
- 卷：46
- 期：5
- 页码：864-869
- 文件大小：504 K
摘要
Flavanone glycosides, such as naringin and neohesperidin, are distributed in some Citrus species and have a chiral center in the C-2 position of the flavanone moiety. Naringin and neohesperidin (2S-form) were separated from the corresponding C-2 epimers (2R-epi-form) by normal-phase HPLC using a polysaccaride-derived chiral stationary phases (CSPs), CHIRALPAK® IB. The analyses of commercial samples of naringin revealed that the relative ratios of naringin to the C-2 epimer were 29–89%. In the case of a commercial sample of neohesperidin, the relative ratio of the neohesperidin (2S-form) is 84%.