Effects of petroleum contamination on soil microbial numbers, metabolic activity and urease activity

详细信息	查看全文 | 推荐本文 |

• 作者：Huan Guo^a ; Jun Yao^a ; ^b ; ^{yaojun@ustb.edu.cn} ; ^{yaojun@cug.edu.cn} ; Minmin Cai^a ; ^c ; Yiguang Qian^b ; Yue Guo^a ; Hans H. Richnow^c ; Ruth E. Blake^d ; Serena Doni^e ; Brunello Ceccanti^e
• 关键词：Total petroleum hydrocarbons ; Soil microbial activity ; Microbial population ; Microcalorimetry ; Urease activity
• 刊名：Chemosphere
• 出版年：2012
• 期刊代码：15_00456535
• 类别：env
• 出版时间：June, 2012
• 卷：87
• 期：11
• 页码：1273-1280
• 文件大小：469 K

摘要

The influence of petroleum contamination on soil microbial activities was investigated in 13 soil samples from sites around an injection water well (Iw-1, 2, 3, 4) (total petroleum hydrocarbons (TPH): 7.5-78 mg kg^鈭?), an oil production well (Op-1, 2, 3, 4, 5) (TPH: 149-1110 mg kg^鈭?), and an oil spill accident well (Os-1, 2, 3, 4) (TPH: 4500-34 600 mg kg^鈭?). The growth rate constant (渭) of glucose stimulated organisms, determined by microcalorimetry, was higher in Iw soil samples than in Op and Os samples. Total cultivable bacteria and fungi and urease activity also decreased with increasing concentration of TPH. Total heat produced demonstrated that TPH at concentrations less than about 1 g kg^鈭? soil stimulated anaerobic respiration. A positive correlation between TPH and soil organic matter (OM) and stimulation of fungi-bacteria-urease at low TPH doses suggested that TPH is bound to soil OM and slowly metabolized in Iw soils during OM consumption. These methods can be used to evaluate the potential of polluted soils to carry out self-bioremediation by metabolizing TPH.