Development and characterisation of chitosan films impregnated with insulin loaded PEG-b-PLA nanoparticles (NPs): A potential approach for buccal delivery of macromolecules
- 作者：Concetta Giovino ; Isaac Ayensu ; John Tetteh ; Joshua S. Boateng ; j.s.boateng@gre.ac.uk ; joshboat@hotmail.com
- 关键词：ASTM ; American Standard Testing Methods ; Ch ; chitosan ; DH ; hydrodynamic diameter ; DLS ; dynamic laser scattering ; NP ; nanoparticle ; PBS ; phosphate buffered saline ; PEG-b-PLA ; poly(ethylene glycol)methyl ether-block-polylactide ; PEG ; polyethylene glycol ; P.
- 刊名：International Journal of Pharmaceutics
- 出版年：2012
- 期刊代码：24_03785173
- 类别：pha
- 出版时间：30 May, 2012
- 卷：428
- 期：1-2
- 页码：143-151
- 文件大小：846 K
摘要
Mucoadhesive chitosan based films, incorporated with insulin loaded nanoparticles (NPs) made of poly(ethylene glycol)methyl ether-block-polylactide (PEG-b-PLA) have been developed and characterised. Blank-NPs were prepared by double emulsion solvent evaporation technique with varying concentrations of the copolymer (5 and 10%, w/v). The optimised formulation was loaded with insulin (model protein) at initial loadings of 2, 5 and 10%with respect to copolymer weight. The developed NPs were analysed for size, size distribution, surface charge, morphology, encapsulation efficiency and drug release. NPs showing negative (味)-potential (<鈭? mV) with average diameter > 300 nm and a polydispersity index (P.I.) of 鈮?.2, irrespective of formulation process, were achieved. Insulin encapsulation efficiencies of 70%and 30%for NPs-Insulin-2 and NPs-Insulin-5 were obtained, respectively. The in vitro release behaviour of both formulations showed a classic biphasic sustained release of protein over 5 weeks which was influenced by pH of the release medium. Optimised chitosan films embedded with 3 mg of insulin loaded NPs were produced by solvent casting with homogeneous distribution of NPs in the mucoadhesive matrix, which displayed excellent physico-mechanical properties. The drug delivery system has been designed as a novel platform for potential buccal delivery of macromolecules.