A simplified hydroethidine method for fast and accurate detection of superoxide production in isolated mitochondria

详细信息	查看全文 | 推荐本文 |

• 作者：Patricia Back ; Filip Matthijssens ; Jacques R. Vanfleteren ; Bart P. Braeckman ; ^{bart.braeckman@ugent.be}
• 关键词：Superoxide ; Hydroethidine ; C. elegans ; Isolated mitochondria
• 刊名：Analytical Biochemistry
• 出版年：2012
• 期刊代码：93_00032697
• 类别：imm
• 出版时间：1 April, 2012
• 卷：423
• 期：1
• 页码：147-151
• 文件大小：553 K

摘要

Because superoxide is involved in various physiological processes, many efforts have been made to improve its accurate quantification. We optimized and validated a superoxide-specific and -sensitive detection method. The protocol is based on fluorescence detection of the superoxide-specific hydroethidine (HE) oxidation product, 2-hydroxyethidium. We established a method for the quantification of superoxide production in isolated mitochondria without the need for acetone extraction and purification chromatography as described in previous studies.