Oxidation reactions of steryl esters in a saturated lipid matrix
- 作者：Mari Lehtonen ; mari.lehtonen@helsinki.fi ; Anna-Maija Lampi ; Mari-Anna Riuttamä ; ki ; Vieno Piironen
- 关键词：CIS-MS ; coordination ion spray mass spectrometry ; DAD ; diode-array detector ; ELSD ; evaporative light-scattering detector ; Fa-OOH ; acyl moiety hydroperoxide ; HPSEC ; high-performance size-exclusion chromatography ; MTBE ; methyl-tert-butyl ether ; PV ; peroxide
- 刊名：Food Chemistry
- 出版年：2012
- 期刊代码：48_03088146
- 类别：abs
- 出版时间：15 October, 2012
- 卷：134
- 期：4
- 页码：2030-2039
- 文件大小：544 K
摘要
In the present study, the formation and further reactions of intact steryl ester hydroperoxides were followed in a tripalmitin matrix maintained at 100 掳C. The effects of the acyl moiety and its unsaturation degree, and of the sterol structure were investigated. Intact steryl ester hydroperoxides were isolated from the lipid matrix by a developed solid-phase extraction (SPE) method and were determined by HPLC-ELSD. Further reactions of hydroperoxides were followed by determining secondary oxidation products of sterol by GC-FID/MS and oligomers by HPSEC-RI. The oxidation of sterol decreased when its solubility in the lipid medium was increased by introduction of acyl moiety. Increased unsaturation of the acyl or steryl moiety of steryl ester shortened the induction period and increased the oxidation of both steryl and acyl moieties. Thus, by changing the chemical and physical properties of sterols, their oxidation may be greatly affected.