Wine extracts from Sardinian grape varieties attenuate membrane oxidative damage in Caco-2 cell monolayers

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• 作者：Monica Deiana^a ; ^{mdeiana@unica.it} ; Debora Loru^a ; ^{dloru@unica.it} ; Alessandra Incani^a ; ^{alessandra.inc@tiscali.it} ; Antonella Rosa^a ; ^{anrosa@unica.it} ; Angela Atzeri^a ; ^{angela.at@tiscali.it} ; Maria Paola Melis^a ; ^{mpmelis@unica.it} ; Barbara Cabboi^a ; ^{barbaracabboi@yahoo.it} ; Laurent Hollecker^b ; ^{laurent_hollecker@hotmail.com} ; Maria Barbara Pinna^c ; ^{barbara.pinna@argiolas.it} ; Francesca Argiolas^c ; ^{francesca.argiolas@argiolas.it} ; Mariano Murru^c ; ^{m.murru@argiolas.it} ; Maria Assunta Dessì ; ^a ; ^{dessima@unica.it}
• 关键词：HP ; fatty acid hydroperoxides ; TBH ; tert-butyl hydroperoxide ; UFA ; unsaturated fatty acids ; MDA ; malondialdehyde ; TER ; transepithelial electrical resistance ; TJ ; tight junctions
• 刊名：Food Chemistry
• 出版年：2012
• 期刊代码：48_03088146
• 类别：abs
• 出版时间：15 October, 2012
• 卷：134
• 期：4
• 页码：2105-2113
• 文件大小：605 K

摘要

One of the most important sites of polyphenol action seems to be in the gastrointestinal system before absorption. We investigated the ability of three wine phenolic extracts, obtained from grape varieties grown in Sardinia, Cannonau (red), Vermentino and Malvasia (white), to exert an antioxidant action against tert-butyl hydroperoxide (TBH)-induced oxidative damage to Caco-2 cell monolayers as a model system of the human intestine. TBH treatment caused the disruption of epithelial integrity, measured as transepithelial electrical resistance, and markers of the peroxidation process of membrane lipids, MDA, fatty acid hydroperoxides and 7-ketocholesterol. All wine extracts were able to counteract the oxidising action of TBH and, in spite of the differences in phenolic composition, exerted a comparable activity. Our findings point out a direct antioxidant action of the wine extracts on enterocytes exposed to oxidising species and further support the opinion that total phenolic content is not essential for antioxidant activity.