The relation between solution association and surface activity of the hydrophobin HFBI from Trichoderma reesei
- 作者：Gé ; za R. Szilvay ; Kaisa Kisko ; Ritva Serimaa ; Markus B. Linder
- 关键词：Hydrophobin ; Protein multimerization ; Protein self-assembly ; Surface active protein
- 刊名：FEBS Letters
- 出版年：2007
- 期刊代码：70_00145793
- 类别：bio
- 出版时间：12 June 2007
- 卷：581
- 期：14
- 页码：2721-2726
- 文件大小：434 K
摘要
Hydrophobins are small fungal surface active proteins that self-assemble at interfaces into films with nanoscale structures. The hydrophobin HFBI from Trichoderma reesei has been shown to associate in solution into tetramers but the role of this association on the function of HFBI has remained unclear. We produced two HFBI variants that showed a significant shift in solution association equilibrium towards the tetramer state. However, this enhanced solution association did not alter the surface properties of the variant HFBIs. The results show that there is not a strong relationship between HFBI solution association state and surface properties such as surface activity.