Identification of Escherichia coli biomarkers responsive to various lignin-hydrolysate compounds

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• 作者：Siseon Lee^a ; Dougu Nam^a ; Joon Young Jung^b ; Min-Kyu Oh^b ; Byoung-In Sang^c ; ^{biosang@hanyang.ac.kr} ; Robert J. Mitchell^a ; ^d ; ^{esgott@unist.ac.kr}
• 关键词：Lignin ; Hydrolysate ; Transcriptome ; Biomarker ; Ferulic acid
• 刊名：Bioresource Technology
• 出版年：2012
• 期刊代码：11_09608524
• 类别：ce
• 出版时间：June, 2012
• 卷：114
• 期：Complete
• 页码：450-456
• 文件大小：523 K

摘要

Aberrations in the growth and transcriptome of Escherichia coli str. BL21(DE3) were determined when exposed to varying concentrations of ferulic acid (0.25-1 g/L), an aromatic carboxylic acid identified within lignin-cellulose hydrolysate samples. The expression of several individual genes (aaeA, aaeB, inaA and marA) was significantly induced, i.e., more than 4-fold, and thus these genes and the heat shock response gene htpG were selected as biomarkers to monitor E. coli鈥檚 responses to five additional hydrolysate-related compounds, including vanillic acid, coumaric acid, 4-hydroxybenzoic acid, ferulaldehyde and furfural. While all of the biomarkers showed dose-dependent responses to most of the compounds, expression of aaeA and aaeB showed the greatest induction (5-30-fold) for all compounds tested except furfural. Lastly, the marA, inaA and htpG genes all showed higher expression levels when the culture was exposed to spruce hydrolysate samples, demonstrating the potential use of these genes as biomarkers.