Anti-inflammatory activity of hydroxycinnamic acid derivatives isolated from corn bran in lipopolysaccharide-stimulated Raw 264.7 macrophages

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• 作者：Eun Ok Kim^a ; Kyoung Jin Min^b ; Taeg Kyu Kwon^b ; Byung Hun Um^c ; Robert A. Moreau^d ; Sang Won Choi^a ; ^{swchoi@cu.ac.kr}
• 关键词：NO ; nitric oxide ; iNOS ; inducible nitric oxide synthase ; NF-魏B ; nuclear factor-kappa B ; LPS ; lipopolysaccharide ; FBS ; fetal bovine serum ; PBS ; phosphate buffered saline ; XTT ; 2 ; 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner
• 刊名：Food and Chemical Toxicology
• 出版年：2012
• 期刊代码：20_02786915
• 类别：pha
• 出版时间：May, 2012
• 卷：50
• 期：5
• 页码：1309-1316
• 文件大小：829 K

摘要

In this study, the effect of the 80%ethanolic extract of corn bran (EECB) on inhibition of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated Raw 264.7 cells was investigated. The EECB inhibited LPS-induced NO production and iNOS expression in a dose-dependent manner. Four hydroxycinnamic acid derivatives (HADs), including two free cinnamic acids, p-coumaric acid (CA) and ferulic acid (FA), and their conjugate phenolic amides, p-dicoumaroyl-putrescine (DCP) and diferuloylputrescine (DFP), were found to be present in the EECB by LC-MS analysis, and DFP (378.66 渭g/g) was the predominant phenolic compound, followed by DCP (7.83 渭g/g) > CA (5.58 渭g/g) > FA (1.84 渭g/g). The four HADs significantly inhibited NO production and iNOS expression in a dose-dependent manner. Among the four HADs tested, DFP showed the most potent inhibition on NO production and iNOS mRNA and protein expression, followed by DCP > FA 猢?#xA0;CA. DFP also exhibited the strongest inhibition on LPS-induced iNOS and NF-魏B luciferase activity, which was followed by DCP 猢?#xA0;FA (CA) > CA (FA). Thus, these results suggest that phenolic amides in the corn bran may be a potential source of natural anti-inflammatory agents.