Sulfated modification of epimedium polysaccharide and effects of the modifiers on cellular infectivity of IBDV

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• 作者：Yu Lu ; Deyun Wang ; Yuanliang Hu ; Xiaoyan Huang ; Junmin Wang
• 关键词：EPS ; Sulfated modification ; IBDV ; Cellular infectivity
• 刊名：Carbohydrate Polymers
• 出版年：2008
• 期刊代码：14_01448617
• 类别：ch
• 出版时间：24 January 2008
• 卷：71
• 期：2
• 页码：180-186
• 文件大小：131 K

摘要

Nine modification conditions were designed to sulfate epimedium polysaccharide (EPS) by chlorosulfonic acid–pyridine method according to orthogonal test, and nine sulfated EPS (sEPS), sEPS₁, sEPS₂, sEPS₃, sEPS₄, sEPS₅, sEPS₆, sEPS₇, sEPS₈ and sEPS₉, were obtained and their effects on IBDV infecting chick embryo fibroblast (CEF) was compared by MTT assay. The results showed that modified sEPS had significantly enhanced CEF resisting IBDV infection in comparison of non-sulfated EPS, especially, sEPS₂ and sEPS₅ presented better effects, which there were certain relationships in the degree of sulfate group (DS) and carbohydrate content. The optimum modification conditions were the reaction temperature of 80 °C, the ratio of chlorosulfonic acid to pyridine of 1:8 and the reaction time of 2 h.