Induction of settlement in larvae of the mussel Mytilus galloprovincialis using neuroactive compounds

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• 作者：Clara Sá ; nchez-Lazo ; ^{clara.sanchez@juntadeandalucia.es} ; Iné ; s Martí ; nez-Pita
• 关键词：Mediterranean mussel ; Potassium
• 刊名：Aquaculture
• 出版年：2012
• 期刊代码：16_00448486
• 类别：abs
• 出版时间：21 May, 2012
• 卷：344-349
• 期：Complete
• 页码：210-215
• 文件大小：342 K

摘要

We investigated the effect on Mytilus galloprovincialis larval settlement, as well as the toxicity, of serial concentrations in filtered seawater of acetylcholine (AC), 纬-aminobutiric acid (GABA); 3-isobutyl-1-methylxanthine (IBMX); and the potassium ion in the form of potassium chloride (KCl) and potassium sulfate (K₂SO₄). All the substances assayed induced larval settlement and peak responses were above 90%in exposures to 10^鈭?#xA0;2 mol L^鈭?#xA0;1 (M) AC, 10^鈭?#xA0;4 and 10^鈭?#xA0;5 M epinephrine, 10^鈭?#xA0;3 M GABA and 20, 30 and 40 mM KCl. The optimal concentration of K⁺ varied depending on the anionic component of the compound assayed, and peak settlement response to KCl was higher (100%) than that achieved with K₂SO₄ (69.7%). The estimated LC50 of the compounds assayed ranged from 9.4 脳 10^鈭?#xA0;6 M (GABA) to 3.1 脳 10^鈭?#xA0;2 M (KCl). GABA, IBMX and K₂SO₄ treatments displayed toxic effects in all the active concentrations. In contrast, AC 10^鈭?#xA0;5 M, epinephrine 10^鈭?#xA0;4 and 10^鈭?#xA0;5 M and KCl 20 mM treatments enhanced larval settlement without an acute short-term effect on mortality. These results provide new insights on the molecular mechanisms controlling settlement in M. galloprovincialis larvae, and yield promising outcomes for the mussel industry to find a reliable method to enhance larval settlement in hatcheries.