BM61 of Bombyx mori nucleopolyhedrovirus: Its involvement in the egress of nucleocapsids from the nucleus

详细信息	查看全文 | 推荐本文 |

• 作者：Hongxing Shen^a ; Keping Chen^b ; ^{kpchen@ujs.edu.cn}
• 关键词：BmNPV ; Bombyx mori nucleopolyhedrovirus ; qPCR ; quantitative polymerase chain reaction ; NPV ; nucleopolyhedrovirus ; GV ; granulovirus ; BV ; budded virus ; ODV ; occlusion-derived virus ; Cm ; chloramphenicol resistance gene ; Hpt ; hour post-transfection ; TCID50 ; t
• 刊名：FEBS Letters
• 出版年：2012
• 期刊代码：70_00145793
• 类别：bio
• 出版时间：5 April, 2012
• 卷：586
• 期：7
• 页码：990-995
• 文件大小：1213 K

摘要

All lepidopteran baculovirus genomes sequenced encode a homolog of the Bombyx mori nucleopolyhedrovirus orf61 gene (Bm61). To determine the role of Bm61 in the baculoviral life cycle, we constructed a Bm61 knockout virus and characterized it in cells. We observed that the Bm61 deletion bacmid led to a defect in production of infectious budded virus (BV). Quantitative PCR analysis of BV in the media culturing the transfected cell indicated that BV was not produced due to Bm61 deletion. Electron microscope analysis showed that in the knockout of Bm61, nucleocapsids were not transported from the nucleus to the cytoplasm. From these results we concluded that BM61 is required in the BV pathway for the egress of nucleocapsids from the nucleus to the cytoplasm.