IL-17A as a regulator of neutrophil survival in nasal polyp disease of patients with and without cystic fibrosis

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• 作者：L. Derycke¹ ; N. Zhang¹ ; G. Holtappels ; T. Dutré ; C. Bachert ; ^{claus.bachert@Ugent.be}
• 关键词：Nasal polyps ; IL-17 ; Neutrophil
• 刊名：Journal of Cystic Fibrosis
• 出版年：2012
• 期刊代码：298_15691993
• 类别：med
• 出版时间：May, 2012
• 卷：11
• 期：3
• 页码：193-200
• 文件大小：914 K

摘要

Nasal polyps in adults are characterized by a chronic inflammation of the upper airways and by the preferential activation of Th2 cells. In contrast, IL-17 producing Th17 cells dominate the inflammation in nasal polyps of cystic fibrosis (CF) patients.

Method

IL-17A, IL-5, IL-6, IL-8, IL-1尾, ECP, MCP-1 and myeloperoxidase expression was determined in tissue homogenates of nasal polyps of non-CF and CF patients and controls. The cellular source of IL-17A was determined by immuno-histochemistry and FACS analysis. The functional role of IL-17A in the survival of neutrophils from CF and non-CF patients was tested.

Results

A significant upregulation of IL-17A and myeloperoxidase could be observed in nasal polyps from CF-patients. The cellular sources of IL-17A in nasal polyps were mainly T-lymphocytes. IL-17A was able to modulate the survival of neutrophils in nasal polyps from non-CF patients; however the survival of neutrophils in CF patients was independent of IL-17A.

Conclusion

The present study shows that IL-17A has an impact on neutrophil survival in adult nasal polyp disease, but not in nasal polyps from CF patients.